Related Experiment Video
Updated: Aug 14, 2026

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
Published on: June 12, 2018
2Apro is a multifunctional protein that regulates the stability, translation and replication of poliovirus RNA
Christy K Jurgens1, David J Barton, Nidhi Sharma
1Department of Biochemistry and Molecular Biology, University of Florida, College of Medicine, Gainesville, 32610-0245, USA.
Abstract:
Poliovirus 2A(pro) is required for the inhibition of host cell protein synthesis and efficient viral replication. We investigated the role of 2A(pro) in regulating viral RNA stability, translation and replication in HeLa S10 reactions. The protease activity of 2A(pro) or its polyprotein precursors, 2AB or P2, was required to increase the stability of viral RNA and prolong translation. Since other viral proteins were not required for the observed effects of 2A(pro), it is likely that a cellular protein(s) modified by 2A(pro) mediated these effects on stability and translation. In addition, the protease activity of 2A(pro) stimulated negative-strand initiation by approximately five-fold but had no effect on positive-strand initiation. The 2A(pro) stimulation of negative-strand synthesis was independent of its effect on stability and translation. These findings further extend the previously known functions of protein 2A(pro) to include its role in increasing RNA stability, prolonging translation and stimulating negative-strand synthesis.
More Related Videos
08:58In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Published on: May 1, 2019
09:12Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
Published on: April 5, 2024
Related Concept Videos
Leaky Scanning
RNA Polymerase II Accessory Proteins
RNA Polymerase II Accessory Proteins
Inhibitors of Virion Maturation and Assembly
Inhibitors of Viral Protein Synthesis
Retrovirus Life Cycles