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Enterococcus faecium low-affinity pbp5 is a transferable determinant.

Louis B Rice1, Lenore L Carias, Susan Rudin

  • 1Medical Service 111(W), Louis Stokes Cleveland VA Medical Center, 10701 East Blvd., Cleveland,Ohio 44106, USA. louis.rice@med.va.gov

Antimicrobial Agents and Chemotherapy
|November 24, 2005
PubMed
Summary
This summary is machine-generated.

Ampicillin resistance in Enterococcus faecium is transferable via the pbp5 gene, even without vancomycin resistance elements. This transfer, occurring between clinical strains, contributes to the spread of ampicillin resistance in U.S. Enterococcus faecium populations.

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Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • Enterococcus faecium is a significant opportunistic pathogen.
  • Ampicillin resistance in E. faecium is often mediated by alterations in the penicillin-binding protein 5 (pbp5).
  • Understanding the mechanisms of resistance gene transfer is crucial for combating antimicrobial resistance.

Purpose of the Study:

  • To investigate the transferability of the ampicillin resistance determinant pbp5 in Enterococcus faecium.
  • To determine if pbp5 transfer occurs independently of vancomycin resistance elements.
  • To explore the genetic context and integration patterns of transferred pbp5.

Main Methods:

  • Conjugation experiments using unrelated Enterococcus faecium isolates as donors and a pbp5-deleted recipient.
  • Quantification of transfer frequencies (transconjugants/recipient CFU).
  • Restriction fragment analysis and mapping of the pbp5 region in clinical isolates.

Main Results:

  • Ampicillin resistance mediated by pbp5 was transferable from most donor strains to the recipient E. faecium.
  • Transfer occurred at low frequencies, suggesting chromosome-to-chromosome transfer.
  • Integration of pbp5 occurred within large genetic regions, often at the site of the previous deletion in the recipient.
  • Distinct genetic patterns upstream of pbp5 were observed between U.S. clinical isolates and strains from turkey feces.

Conclusions:

  • The pbp5 gene conferring ampicillin resistance is a transferable determinant in Enterococcus faecium.
  • pbp5 transfer can occur independently of vancomycin resistance mobile elements.
  • The transfer of pbp5 between clinical isolates contributes to the dissemination of ampicillin resistance in U.S. Enterococcus faecium strains.