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Updated: Aug 14, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Synthesis of proteins by native chemical ligation using Fmoc-based chemistry
Julio A Camarero1, Alexander R Mitchell
1Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA. camarero1@llnl.gov
Abstract:
C-terminal peptide alpha-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the chemical synthesis of peptide alpha-thioesters using Fmoc-based SPPS.

