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Updated: Aug 14, 2026
06:43
In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
Published on: September 12, 2025
Intein-mediated, in vitro and in vivo protein modifications with small molecules
1Department of Biological Sciences, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.
Protein and Peptide Letters
|November 25, 2005
Abstract:
We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.
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02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
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01:26Protein Modifications in the RER
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01:24Tagging and Fusion Proteins
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