Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrated expressional analysis: application to the drug discovery process.

Sergey E Ilyin1, Daniel Horowitz, Stanley M Belkowski

  • 1Johnson & Johnson Pharmaceutical Research & Development, L.L.C. Spring House, PA 19477, USA.

Methods (San Diego, Calif.)
|November 26, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stroke-induced immunosuppression is associated with brain injury and poor outcomes in patients receiving intravenous thrombolysis: A multicenter prospective cohort study.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026
Same author

Non-pharmacological therapies for breast cancer-related lymphedema: a systematic review and network meta-analysis based on randomized controlled trials.

Frontiers in oncology·2026
Same author

Factors Influencing Transition Readiness in Adolescents with Systemic Lupus Erythematosus: A Qualitative Study.

Patient preference and adherence·2026
Same author

Protocol for isolating cells from a single cryopreserved gastrointestinal endoscopic biopsy for single-cell RNA sequencing.

STAR protocols·2026
Same author

Arginine protects against colonic barrier injury induced by long-term peritoneal dialysis.

Scientific reports·2026
Same author

The LIF-LIFR Axis Promotes Liver Regeneration via Modulation of Angiogenesis and HGF Release from LSECs.

bioRxiv : the preprint server for biology·2026

This study presents an integrated assay strategy for CNS drug discovery, combining high-content imaging with multiplexed RT-PCR and protein analysis. This approach enhances the validation of microarray findings for novel therapeutic applications and biomarker identification.

Area of Science:

  • Neuroscience
  • Drug Discovery
  • Molecular Biology

Background:

  • Microarray technology facilitates high-throughput gene expression analysis for neuroscience research.
  • Validating microarray results and discovering novel therapeutic targets requires scalable, integrated assay methods.
  • Central nervous system (CNS) drug discovery necessitates understanding target biology, disease pathophysiology, lead compound selection, and ADME/toxicity profiles.

Purpose of the Study:

  • To establish a novel screening strategy integrating high-content imaging and quantitative mRNA analysis for CNS drug discovery.
  • To create an interface between high-throughput microarray data and specific marker validation.
  • To demonstrate the utility of this integrative approach for target and biomarker identification, and toxicity studies.

Main Methods:

Related Experiment Videos

  • Combined high-content image-based testing (Array Scan) with confocal microscopy and multiplexed TaqMan RT-PCR for quantitative mRNA expression.
  • Utilized the same cell population for both image-based assays and subsequent mRNA analysis.
  • Incorporated Ciphergen ProteinChip analysis of the supernatant for complementary protein data.

Main Results:

  • Successfully demonstrated sequential testing of cell populations for imaging and mRNA analysis.
  • Achieved multiplexing of different targets within a single real-time PCR tube.
  • Showcased the integration of imaging, mRNA, and protein analysis for comprehensive data output.

Conclusions:

  • The developed integrative technological approach is valuable for CNS drug discovery.
  • This strategy facilitates target and biomarker identification, selection, and characterization.
  • The approach aids in studying molecular mechanisms associated with toxicity and adverse events.