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Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression
Claudia S Petit1, Sapna Mehta, Rachel H Roberts
1Howard Hughes Medical Institute, and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Journal of Cell Science
|December 1, 2005
Summary
Sep1p and Ace2p transcription factors control Mid2p production, which is essential for septin ring assembly in fission yeast cell division. Overproduction of these factors disrupts septin ring disassembly.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Fission yeast cell division involves a contractile ring and septin ring.
- Mid2p is crucial for septin ring formation during cell division.
- Ace2p and Sep1p are transcription factors involved in cell division.
Purpose of the Study:
- Investigate the regulatory pathway controlling Mid2p production.
- Determine the roles of Sep1p and Ace2p in septin ring assembly and disassembly.
- Elucidate the function of Ace2p during cell division.
Main Methods:
- Gene expression analysis (microarray).
- Genetic analysis of transcription factor function.
- Protein localization and modification studies (phosphorylation, ubiquitylation).
Main Results:
- Mid2p production is regulated by Ace2p, which acts downstream of Sep1p.
- Sep1p and Ace2p are required for septin ring assembly.
- Overexpression of Sep1p or Ace2p inhibits septin ring disassembly.
- Ace2p levels peak in anaphase and undergo post-translational modification.
Conclusions:
- Sep1p and Ace2p form a regulatory cascade controlling septin ring formation for efficient cell division.
- Ace2p functions independently of the septation initiation network.
- Temporal regulation of Ace2p is critical for proper cell division progression.