Related Experiment Video
Updated: Aug 14, 2026
Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
Published on: September 14, 2017
[Isolation, induction and differentiation of human blood-derived endothelia progenitor cells]
Ze-Hao Liu1, Min-Xiang Lei, Ai-Min Wang
1Departement of Endocrinology, Xiangya Hospital, Xiangya Hospital, Central South University, Changsha, China. medicalliu@hotmail.com
Objective:
To determine the biological traits and optimal condition for the induction and differentiation of endothelial progenitor cells from peripheral blood in healthy adults.
Methods:
Mononuclear cells isolated from peripheral blood of healthy adults were cultured in M199 medium supplemented with VEGF, bFGF, IGF-1, and EGF. The appearing time of cell clusters or spindle-shaped cells was recorded respectively. Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelial-specific markers.
Results:
Attached spindle-like cells appeared 4 days after the culture, cell clusters were observed at 5 to 8 days, and cord-like structure was formed by 10th day. These cells expressed endothelial-specific markers.
Conclusion:
Endothelial progenitors cells were derived from mononuclear cells of peripheral blood, which can be induced into endothelial cells at specific conditions.
