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Competitive Genomic Screens of Barcoded Yeast Libraries
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Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Biochemical and genetic analysis of the yeast proteome with a movable ORF collection

Daniel M Gelperin1, Michael A White, Martha L Wilkinson

  • 1Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.

Genes & Development
|December 3, 2005
PubMed

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...