Related Experiment Video
Updated: Aug 14, 2026
Model of Ischemia and Reperfusion Injury in Rabbits
Published on: November 3, 2023
Localization and changes of diamine oxidase during cardiopulmonary bypass in rabbits
Nobuo Tsunooka1, Kazutaka Maeyama, Hiromichi Nakagawa
1Second Department of Surgery, Ehime University School of Medicine, Ehime, Japan. tsunooka@m.ehime-u.ac.jp
Background:
We previously observed increased serum diamine oxidase activity during clinical cardiopulmonary bypass, indicating small intestinal mucosal ischemia followed by bacterial translocation.
Materials And Methods:
In seven female rabbits undergoing cardiopulmonary bypass for 1 h, we analyzed the localization of diamine oxidase immunohistochemically, and measured its activity in serum and abdominal organs before and after cardiopulmonary bypass (CPB).
Results:
Preoperatively, diamine oxidase activity and immunoreactivity were high in the small intestine, localized to villus tips. Serum activity increased significantly after CPB, whereas small intestinal diamine oxidase decreased with mucosal injury.
Conclusions:
In this model serum diamine oxidase activity appeared to reflect CPB induced intestinal mucosal injury.
