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Sterilisation of extracted human teeth for educational use
M Kumar1, P S Sequeira, S Peter
1Department of Microbiology, Kasturba Medical College, Mangalore-575 001, Karnataka, India.
Indian Journal of Medical Microbiology
|December 6, 2005
Summary
Autoclaving and 10% formalin effectively sterilize extracted human teeth. Other methods like hydrogen peroxide, sodium hypochlorite, and boiling were found ineffective for dental infection control.
Area of Science:
- Dentistry
- Microbiology
Background:
- Extracted human teeth are utilized in dental education and research.
- Proper sterilization of these teeth is crucial to prevent infection transmission.
Purpose of the Study:
- To evaluate the efficacy of different sterilization methods for extracted human teeth.
- To assess dental students' awareness of infection risks and disinfection protocols.
Main Methods:
- Sixty extracted teeth were divided into six groups and subjected to different treatments: 10% formalin, 3% hydrogen peroxide, 2.6% sodium hypochlorite, boiling, autoclaving, and saline immersion.
- Post-treatment, teeth were cultured to assess microbial contamination.
- A questionnaire surveyed dental students on infection awareness and sterilization practices.
Main Results:
- Autoclaving (121°C, 15 psi, 30 min) and 10% formalin immersion (7 days) proved effective in sterilizing extracted teeth.
- 3% hydrogen peroxide, 2.6% sodium hypochlorite, and boiling water were ineffective sterilization methods.
- Survey revealed varying levels of student awareness regarding infection control with extracted teeth.
Conclusions:
- Autoclaving or 10% formalin immersion are recommended for effective disinfection and sterilization of extracted human teeth.
- Standardization of sterilization protocols is necessary in dental education.
- Further research may explore alternative, rapid sterilization techniques.