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Updated: Aug 14, 2026

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
Published on: July 23, 2013
Production of a recombinant bacterial lipoprotein in higher plant chloroplasts
Karin Glenz1, Bernadette Bouchon, Thomas Stehle
1Department of Pharmaceutical Biology, Julius-von-Sachs-Institut für Biowissenschaften, Julius-Maximilians Universität Wuerzburg, Julius-von-Sachs-Platz 2, 97082 Wuerzburg, Germany.
Abstract:
Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme disease. The recombinant OspA exhibits a lipid modification typical for bacteria and induced protective antibodies in mice, demonstrating that functionally active bacterial lipoproteins can be processed in plants.
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