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Updated: Aug 14, 2026
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Published on: July 5, 2019
Heterogeneous t(4;11) fusion transcripts in two infants with acute lymphoblastic leukemia
Harvindar Kaur Gill1, Sew Keoh Ten, Jasbir Singh Dhaliwal
1Allergy & Immunology Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia. gillhk@imr.gov.my
Abstract:
An RT-PCR assay detected the t(4;11) translocation in two infants with acute lymphoblastic leukemia (ALL). Case P76 was a 10-month-old, female infant, who presented with a WBC of 137.4 x 10(9)/l and a pre-pre-B ALL immunophenotype. Case P120 was a 6-month-old female infant, with a WBC > 615 x 10(9)/l and a pre-pre-B ALL immunophenotype. RT-PCR of cDNA from both these cases generated a 656 bp and a 542 bp respectively, which sequencing confirmed as t(4;11) fusion transcripts. The primers and conditions selected for this assay are compatible with a one-step multiplex PCR for the main translocations in childhood ALL.
