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Published on: August 13, 2016
Glucose regulated proteins 78 and 75 bind to the receptor for hyaluronan mediated motility in interphase microtubules
Hiroko Kuwabara1, Masahiko Yoneda, Hana Hayasaki
1Department of Pathology, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan. pa2020@art.osaka-med.ac.jp
Abstract:
The receptor for hyaluronan mediated motility (RHAMM), which is a hyaluronan-binding protein, is a centrosomal and microtubal protein. Here, we have identified two RHAMM-binding proteins, glucose regulated protein (GRP) 78 and GRP75, using co-immunoprecipitation analysis. These two proteins directly bound to glutathione-S-transferase-RHAMM fusion proteins. By double immunostaining, GRP78 and GRP75 colocalized with RHAMM in interphase microtubules, but were separated in mitotic spindles. Prevention of microtubule polymerization by TN-16 and vincristine sulfate induced RHAMM overexpression without a significant change in GRP78/75. Taken together, GRP78/75 and RHAMM complexes may stabilize microtubules in the interphase, associated with a downregulation of RHAMM. These results reveal a new biochemical activity of RHAMM.
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