Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New universal primers facilitate Pyrosequencing.

Atakan Aydin1, Mohammad R Toliat, Sylvia Bähring

  • 1Franz Volhard Clinic, HELIOS Kliniken-Berlin and Max Delbrück Center for Molecular Medicine, Medical Faculty of the Charité, Humboldt University of Berlin, Berlin, Germany.

Electrophoresis
|December 7, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potential impact on cholesterol goal achievement and predicted cardiovascular risk by the addition of ezetimibe and bempedoic acid on top of statins: a simulation from the SANTORINI study.

American journal of preventive cardiology·2026
Same author

[Acute lung failure].

Pneumologie (Stuttgart, Germany)·2026
Same author

Real-World Effectiveness of Bempedoic Acid in Reducing LDL-C: An Electronic Medical Records-Based Analysis.

JACC. Advances·2026
Same author

A biallelic MRPL42 variant causes a combined oxidative phosphorylation deficiency syndrome revealed by multi-omics.

NPJ genomic medicine·2026
Same author

Lipid management in high cardiovascular risk patients in France: a comparison with rest of Europe patients from 1-year follow-up of SANTORINI.

Frontiers in cardiovascular medicine·2026
Same author

A bioinspired microdevice unifying energy storage and actuation through hydration control.

Nature communications·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

New universal primers simplify Pyrosequencing by enabling biotin introduction during PCR, reducing costs. This method maintains high pyrogram quality and adds flexibility to genotyping assay design.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Pyrosequencing costs are often driven by the requirement for single-stranded PCR products.
  • Traditional methods necessitate biotin-labeling of a primer, adding complexity and expense.

Purpose of the Study:

  • To develop novel universal primers for simplified biotin introduction in PCR.
  • To reduce the overall cost of Pyrosequencing assays.

Main Methods:

  • Designed universal primers allowing biotin introduction at either forward or reverse primer in a single PCR reaction.
  • Converted five human single nucleotide polymorphism (SNP) assays to the universal primer format.
  • Evaluated pyrogram quality and assay establishment success.

Main Results:

Related Experiment Videos

  • Achieved similar high-quality pyrograms compared to standard methods.
  • Demonstrated flexibility in assay design with optional strand selection.
  • Facilitated cost reduction in genotyping through streamlined primer design.

    • Conclusions:

    • The developed universal primer system offers a more flexible and cost-effective approach to Pyrosequencing.
    • While proprietary software was often needed for assay establishment, the method shows significant promise for reducing genotyping costs.