Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly efficient constant-current electroporation increases in vivo plasmid expression.

Amir S Khan1, Melissa A Pope, Ruxandra Draghia-Akli

  • 1ADViSYS, Inc., The Woodlands, TX 77381, USA.

DNA and Cell Biology
|December 8, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Menaquinone depletion resensitises bedaquiline-resistant tuberculosis.

bioRxiv : the preprint server for biology·2026
Same author

The Indispensable Value of Small-Molecule Antivirals in Epidemic and Pandemic Preparedness.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2025
Same author

A small-molecule SARS-CoV-2 inhibitor targeting the membrane protein.

Nature·2025
Same author

Antiviral target compound profile for pandemic preparedness.

Nature reviews. Drug discovery·2024
Same author

Overview of ACTIV trial-specific lessons learned.

Journal of clinical and translational science·2024
Same author

Preparing better: Accelerating COVID-19 Therapeutic Interventions and Vaccines (ACTIV) therapeutics trials lessons learned: A call to the future.

Journal of clinical and translational science·2024
Same journal

Fibroblast Growth Factor 18 Regulates Mitochondrial Function to Alleviate Myocardial Ischemia-Reperfusion Injury in Rats.

DNA and cell biology·2026
Same journal

Silencing Long Noncoding RNA <i>CRNDE</i> Alleviates Hypoxia-Induced Myocardial Cell Injury by Inhibiting the NLRP3/ASC Pathway.

DNA and cell biology·2026
Same journal

Integrated Multi-Omics Profiling Reveals Coordinated Transcriptional, Translational, and Metabolic Remodeling in IPF Fibroblasts.

DNA and cell biology·2026
Same journal

The Innate Immune Protein IFITM3 as a γ-Secretase Modulatory Protein: From Inflammation to Alzheimer's Disease.

DNA and cell biology·2026
Same journal

Glycosyltransferases as Oncogenic Drivers: Lessons from Cancer Genome Mining.

DNA and cell biology·2026
Same journal

Essential Role for XRCC1 and PARP1 in the Repair of Genotoxic Damage During Spermatogenesis.

DNA and cell biology·2026
See all related articles

Constant-current electroporation optimizes DNA delivery into muscle tissue. Tailoring pulse parameters to species-specific tissue resistance enhances plasmid expression and minimizes damage.

Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Gene Therapy

Background:

  • Electroporation enhances plasmid delivery for DNA vaccines and therapeutic proteins into skeletal muscle.
  • Constant-voltage electroporation methods can cause tissue damage and reduce plasmid expression due to uncompensated tissue resistance.

Purpose of the Study:

  • To investigate the efficacy of software-driven constant-current electroporation for plasmid delivery in mice and pigs.
  • To identify optimal electroporation parameters based on species-specific tissue resistance and age.

Main Methods:

  • Utilized a constant-current electroporator to deliver plasmids to mice and pigs (small and large).
  • Recorded and analyzed tissue voltage, amperage, and resistance during electroporation pulses.
  • Determined optimal amperage, pulse length, and lag time for mice, and evaluated pulse patterns.

Related Experiment Videos

Main Results:

  • Optimal electroporation conditions were species- and age-dependent, correlating with tissue resistance.
  • Pigs exhibited lower muscle resistance than mice, with younger pigs having higher resistance than older pigs.
  • Identified optimal parameters for mice: 0.1 Amps, 20 msec pulse length, and 0 sec lag time. Electroporation pulse patterns also influenced expression.

Conclusions:

  • Constant-current electroporation offers improved plasmid delivery compared to constant-voltage methods.
  • Optimizing electroporation parameters, including pulse pattern and considering age- and tissue-specific resistance, is crucial for maximizing plasmid expression in different species.