Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Understanding dioxin developmental toxicity using the zebrafish model.

Sara A Carney1, Amy L Prasch, Warren Heideman

  • 1Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin 53705-2222, USA.

Birth Defects Research. Part A, Clinical and Molecular Teratology
|December 8, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Influence of Primary Ion Species and Cluster Size on the Time-of-Flight Secondary Ion Mass Spectrum of Ciprofloxacin in Water Ice.

Analytical chemistry·2026
Same author

The influence of sample temperature on water cluster ion formation for ToF-SIMS studies of frozen hydrated samples.

Analytical and bioanalytical chemistry·2025
Same author

Laser Secondary Neutral Mass Spectrometry of Bi<sub>3</sub><sup>+</sup> Sputtered Pharmaceuticals.

Analytical chemistry·2025
Same author

Strategies for Minimizing Interference from Metastable Water Clusters in ToF-SIMS 3D Imaging of Frozen Hydrated Biological Samples.

Journal of the American Society for Mass Spectrometry·2025
Same author

Time-of-flight secondary ion mass spectrometry investigation of the influence of ambient air exposure on the migration of brain lipids in a matrix commonly used in matrix assisted laser desorption ionization mass spectrometry imaging.

Biointerphases·2025
Same author

Mass Spectrometry Imaging with Matrix-Enhanced SIMS: The Influence of Matrix Deposition Rate on Crystal Size and Signal Enhancement.

Analytical chemistry·2025

Zebrafish larvae exposed to dioxin (TCDD) exhibit developmental toxicity, including "blue sac syndrome." This toxicity is mediated by the aryl hydrocarbon receptor (AHR) and ARNT signaling pathway, specifically zfAHR2 and zfARNT1.

Area of Science:

  • Toxicology
  • Developmental Biology
  • Aquatic Toxicology

Background:

  • Zebrafish (Danio rerio) are valuable models for developmental toxicity studies.
  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent environmental contaminant known to cause developmental toxicity.
  • TCDD-induced developmental toxicity in zebrafish larvae, termed "blue sac syndrome," includes edema, anemia, and cardiovascular and craniofacial malformations.

Purpose of the Study:

  • To investigate the role of the aryl hydrocarbon receptor (AHR)/aryl hydrocarbon receptor nuclear translocator (ARNT) signaling pathway in mediating TCDD developmental toxicity in zebrafish.
  • To elucidate the molecular mechanisms underlying TCDD-induced developmental defects in zebrafish.

Main Methods:

  • Utilized morpholino-based knockdown of zfAHR1, zfAHR2, zfARNT1, and zfARNT2 mRNAs to assess their role in TCDD toxicity.

Related Experiment Videos

  • Employed a zfARNT2 null mutant zebrafish line for further mechanistic insights.
  • Investigated the involvement of cytochrome P4501A (zfCYP1A) in TCDD toxicity.

    • Main Results:

    • zfAHR2 and zfARNT1 were identified as the key mediators of TCDD developmental toxicity in zebrafish.
    • Knockdown of zfAHR2 or zfARNT1 significantly altered TCDD-induced developmental endpoints.
    • TCDD developmental toxicity is not mediated by increased zfCYP1A expression.

    Conclusions:

    • The AHR/ARNT pathway, specifically zfAHR2 and zfARNT1, plays a critical role in mediating TCDD developmental toxicity in zebrafish.
    • These findings advance the understanding of dioxin toxicity mechanisms at the molecular level.
    • Zebrafish remain a powerful model for studying the developmental effects of environmental contaminants.