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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

Molecular biology and mutation of green fluorescent protein

David A Zacharias1, Roger Y Tsien

  • 1The Whitney Laboratory for Marine Bioscience, University of Florida Department of Neuroscience, St. Auqustine, FL, USA.

Methods of Biochemical Analysis
|December 13, 2005
PubMed
Abstract

No abstract available in PubMed .

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...