Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human osteoblasts produce cathepsin K.

Jami Mandelin1, Mika Hukkanen, Tian-Fang Li

  • 1Institute of Biomedicine/Anatomy, Biomedicum Helsinki, PO Box 63, FI-00014 University of Helsinki, Finland. jami.mandelin@helsinki.fi

Bone
|December 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient NK cell transduction with VSV-G-pseudotyped lentiviral vectors.

Molecular therapy. Advances·2026
Same author

Protocol for intracardiac delivery and live imaging of NK-tumor interactions in an ex ovo chick embryo model.

STAR protocols·2026
Same author

Multimodal immunopharmacologic screens identify drugs rewiring the cancer-immune interface.

bioRxiv : the preprint server for biology·2026
Same author

In vitro muscle contraction: A technical review on electrical pulse stimulation in C2C12 cells.

Experimental physiology·2025
Same author

ATF-4 deficiency increases ER stress and induces osteoarthritis formation in mice.

Molecular and cellular biochemistry·2025
Same author

Improved Translational Relevance of In Vitro Fibrosis Models by Integrating IOX2-Mediated Hypoxia-Mimicking Pathways.

Biomedicines·2025

Osteoblasts produce cathepsin K, an enzyme crucial for bone remodeling. This finding suggests osteoblasts play a direct role in maintaining and recycling the bone

Area of Science:

  • Bone Biology
  • Biochemistry
  • Cell Biology

Background:

  • Bone remodeling is a continuous process involving osteoclasts and osteoblasts.
  • Osteoclasts use cathepsin K (a cysteine proteinase) to degrade the organic bone matrix.
  • Recent evidence suggests osteoblasts may also contribute to organic matrix remodeling.

Purpose of the Study:

  • To investigate the production of cathepsin K by osteoblasts.
  • To determine if osteoblasts contribute to organic bone matrix remodeling through cathepsin K secretion.

Main Methods:

  • Immunohistochemistry on human trabecular bone sections.
  • Isolation and culture of osteoblast-like cells from bone samples.
  • Western blotting and quantitative RT-PCR to analyze cathepsin K expression and production.

Related Experiment Videos

Main Results:

  • Osteoblasts, bone lining cells, and osteocytes exhibited cathepsin K immunoreactivity in situ.
  • Osteoblast-like cells in vitro produced cathepsin K mRNA.
  • These cells released both pro-cathepsin K (42 kDa) and processed cathepsin K (27 kDa) into culture media.

Conclusions:

  • Osteoblasts synthesize and secrete cathepsin K, indicating a direct role in organic matrix maintenance.
  • Osteoblastic cathepsin K may be involved in recycling improperly processed collagen I.
  • Further research is needed to explore the implications of osteoblastic cathepsin K in bone diseases.