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Updated: Aug 14, 2026

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Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Fusion-based strategies to identify genes involved in degradation of a specific substrate
1UCSD Division of Biologcal Sciences, La Jolla, California, USA.
Methods in Enzymology
|December 13, 2005
Abstract:
Fusion proteins have been used in many instances to allow genetic screening for genes required for the degradation of a specific substrate. This straightforward, yet powerful, approach can be applied in many circumstances to facilitate gene characterization and discovery. Some general principles are discussed and then several successful uses of these tactics are described in detail.

