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Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X-100
M A Partearroyo1, M A Urbaneja, F M Goñi
1Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
FEBS Letters
|May 11, 1992
Abstract:
Effective detergent:lipid ratios (i.e. molar ratios in the mixed aggregates, vesicles or micelles) have been estimated for the solubilization of phosphatidylcholine vesicles by Triton X-100. Effective molar ratios are given for both the onset and the completion of bilayer solubilization; small unilamellar, large unilamellar and multilamellar vesicles have been used. Effective detergent:lipid ratios are independent of phospholipid concentration, and their use allows a deeper understanding of membrane-surfactant interactions.