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[HPLC fingerprint of Fritillaria hupehensis]
Guo-Xin Zhang1, Juan Li, Peng Zhang
1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Aim:
To establish a fingerprint analysis method of Fritillaria hupehensis.
Methods:
fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL x min(-1); recording time was 60 min; drift tube temperature was 75 degrees C; gas flow rate was 1.9 L x min(-1).
Results:
HPLC fingerprint of Fritillaria hupehensis was obtained.
Conclusion:
A reliable method was provided for controlling the quality of Fritillaria hupehensis.