Comparison between sample disruption methods and solid-liquid extraction (SLE) to extract phenolic compounds from...

Area of Science:

• Phytochemistry
• Analytical Chemistry
• Natural Product Extraction

Background:

• Phenolic compounds in Ficus carica leaves possess significant biological activities.
• Efficient extraction methods are crucial for isolating these valuable compounds.
• Traditional methods like solid-liquid extraction (SLE) can be time-consuming and less efficient.

Purpose of the Study:

• To compare the efficacy of Sea Sand Disruption Method (SSDM) and Matrix Solid Phase Disruption (MSPD) against Solid-Liquid Extraction (SLE) for phenolic compound extraction.
• To evaluate the yield, reproducibility, cost, and environmental impact of different extraction techniques.
• To identify the optimal method for extracting specific phenolic compounds from Ficus carica leaves.

Main Methods:

• Solid-Liquid Extraction (SLE)
• Sea Sand Disruption Method (SSDM)
• Matrix Solid Phase Disruption (MSPD)
• Analysis of variance (ANOVA) for statistical comparison of extraction yields.

Main Results:

• SSDM and MSPD yielded significantly higher amounts of phenolic compounds compared to SLE.
• SSDM demonstrated superior reproducibility (RSD < 5%) and cost-effectiveness.
• High recovery rates (>85%) were achieved for chlorogenic acid, rutin, and psoralen using SSDM.

Conclusions:

• Solid disruption methods, particularly SSDM, are more effective and efficient for extracting phenolic compounds from Ficus carica leaves.
• SSDM offers a faster, ecologically friendly, reproducible, and economical alternative to traditional extraction techniques.
• The findings support the use of SSDM for the isolation of specific bioactive compounds like chlorogenic acid, rutin, and psoralen.