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Updated: Jun 24, 2026
11:39
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Sugar level, fermentability, and acceptability of straw treated with different acids
C J Israilides1, G A Grant, Y W Han
1Department of Microbiology, Oregon State University, Corvallis, Oregon 97331.
Applied and Environmental Microbiology
|July 1, 1978
Summary
Ryegrass straw treated with phosphoric acid (H3PO4) showed the best vole acceptability. Combining HCl and H3PO4, followed by Aureobasidium pullulans fermentation, maximized straw palatability and fermentability.
Area of Science:
- Agricultural Science
- Biotechnology
- Animal Nutrition
Background:
- Ryegrass straw is an abundant lignocellulosic material.
- Acid treatment can enhance the digestibility and palatability of straw for animal feed.
- Optimizing acid treatment is crucial for improving straw utilization.
Purpose of the Study:
- To evaluate the effects of different acid treatments on ryegrass straw.
- To assess sugar release, fermentability, and vole acceptability of treated straw.
- To determine optimal conditions for enhancing straw's nutritional value.
Main Methods:
- Ryegrass straw was treated with hydrochloric acid (HCl) and phosphoric acid (H3PO4) individually and in combination.
- Sugar release was quantified.
- Fermentability was assessed using Candida utilis and Aureobasidium pullulans.
- Vole acceptability trials were conducted.
Main Results:
- HCl treatment yielded the highest sugar release, but H3PO4 resulted in the best vole acceptability.
- Candida utilis growth was inhibited on straw treated with 0.5 N HCl, likely due to high chloride levels or sugar degradation products.
- A combination of 0.23 N HCl and 0.15 N H3PO4 maximized straw fermentability and palatability.
- Aureobasidium pullulans fermentation further improved the palatability of acid-treated straw.
Conclusions:
- Phosphoric acid treatment is most effective for improving ryegrass straw palatability for voles.
- Combined acid treatment (HCl and H3PO4) followed by Aureobasidium pullulans fermentation offers a promising strategy for enhancing straw utilization.
- Further research is needed to understand the specific inhibitory mechanisms of high HCl concentrations on microbial growth.
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