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Updated: Jul 10, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Medium for enhanced growth of bacteria from a Swine manure digester
E L Iannotti1, J R Fischer, D M Sievers
1Department of Agricultural Engineering, University of Missouri-Columbia, and Science and Education Administration, U.S. Department of Agriculture, Columbia, Missouri 65211.
Abstract:
Most bacterial strains isolated from a swine manure digester grew sufficiently to permit transfer of cultures, but not characterization. Substrates, crude extracts, growth factors, and electron acceptors were evaluated for growth promotion. The growth of all but one group of the isolates was substantially increased with a medium containing glucose, cellobiose, soluble starch, pyruvate, peptone, yeast extract, minerals, volatile acids, vitamins, hemin plus vitamins K(1) and K(3), sodium bicarbonate, cysteine, and digester fluid. The strains require both known and unknown factors (in crude extracts) for maximal growth.
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