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Updated: Aug 14, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Structure-activity relationships in microbial transformation of phenols
D F Paris1, N L Wolfe, W C Steen
1Environmental Research Laboratory, U.S. Environmental Protection Agency, Athens, Georgia 30613.
Abstract:
The second-order rate constants for the microbial transformation of a series of phenols were correlated with the physicochemical properties of the phenols. The compounds studied were phenol, p-methylphenol, p-chlorophenol, p-bromophenol, p-cyanophenol, p-nitrophenol, p-acetylphenol, and p-methoxyphenol. Phenol-grown cells of Pseudomonas putida U transformed these compounds. Microbial transformation rate constants ranged from (1.5 +/- 0.99) x 10 liter . organism . h for p-cyanophenol to (7.0 +/- 1.3) x 10 liter . organism . h for phenol. Linear regression analyses of rate constants and electronic, steric, and hydrophobic parameters showed that van der Waal's radii gave the best coefficient of determination (r = 0.956). Products identified by thin-layer chromatography and liquid chromatography indicated that the phenols were microbially oxidized to the corresponding catechols.
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