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Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
Published on: February 7, 2017
Method for rapid detection of cyanogenic bacteria
1Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282.
Applied and Environmental Microbiology
|February 1, 1983
Abstract:
An agar plate method is described in which the production of hydrogen cyanide by as many as 50 microbial isolates per plate may be detected. Cyanide produced by the organisms reacts with copper(II) ethylacetoacetate and 4,4'-methylenebis-(N,N-dimethylaniline) in a paper disk suspended above the microbial colonies. Cell growth occurs in depressions in the agar surface, which allows separation of colonies and enhances sensitivity of hydrogen cyanide detection.
