Related Experiment Video
Updated: Sep 3, 2026
Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Leptospira spp. Toolbox for Chemotaxis Assay
1ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des matériaux I-MAT (FD4122), Université de Cergy-Pontoise, Maison Internationale de la Recherche (MIR), rue Descartes,, 95001, Neuville sur Oise Cedex, France. ambroise.lambert@cyu.fr.
Abstract:
A toolbox for chemotaxis assay adapted to Leptospira spp. has emerged in the recent years: soft agar assay, capillary assay, and videomicroscopy tracking. Those methods allow to demonstrate chemotaxis defect, identify diverse chemoattractant, or decipher motile behavior quantitatively. These experiments have demonstrated a role of motility and potentially chemotaxis in leptospirosis pathogenesis. We describe extensively the methods and provide the key steps to use this toolbox.
