Expression of a Lactose Transposon (Tn951) in Zymomonas mobilis
V C Carey1, S K Walia, L O Ingram
1Department of Microbiology and Cell Science and Department of Immunology and Medical Microbiology, University of Florida, Gainesville, Florida 32611.
Abstract:
The potential utility of Zymomonas mobilis as an organism for the commercial production of ethanol would be greatly enhanced by the addition of foreign genes which expand its range of fermentable substrates. We tested various plasmids and mobilizing factors for their ability to act as vectors and introduce foreign genes into Z. mobilis CP4. Plasmid pGC91.14, a derivative of RP1, was found to be transferred from Escherichia coli to Z. mobilis at a higher frequency than previously reported for any other plasmids. Both tetracycline resistance and the lactose operon from this plasmid were expressed in Z. mobilis CP4. Plasmid pGC91.14 was stably maintained in Z. mobilis at 30 degrees C but rapidly lost at 37 degrees C.
Related Concept Videos
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
Transposons
Operons
Overview of Transposition and Recombination
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms


