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Partial Purification and Characterization of a Polysaccharide Depolymerase Associated with Phage-Infected Erwinia
P A Vandenbergh1, A M Wright, A K Vidaver
1Microlife Technics, 1833 57th St., Sarasota, Florida 34243, and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583.
Abstract:
Erwinia amylovora infected with bacteriophage ERA103 produced an enzyme which degraded the extracellular polysaccharide of noninfected cells. The depolymerase enzyme was purified 15-fold by a procedure which included ammonium sulfate precipitation, ultracentrifugation, CM-Sephadex batchwise separation, Sephadex G-50 column chromatography, and Sephacryl S-200 column chromatography. The enzyme had a molecular weight of approximately 21,000 and a pH optimum of 6.0. Activity was enhanced by supplements of 2-mercaptoethanol or dithiothreitol.
