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Minimum threshold for hydrogen metabolism in methanogenic bacteria.
1Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061.
Applied and Environmental Microbiology
|June 1, 1985
Summary
Pure cultures of methanogens require high hydrogen levels (6.5 Pa) for consumption. These findings support the threshold model for methane production in sediments, contradicting prior research.
Area of Science:
- Microbiology
- Environmental Science
- Geochemistry
Background:
- Methane production in sediments is crucial for carbon cycling.
- The threshold model proposes a minimum hydrogen level for methanogenesis.
- Previous studies suggested methanogens could consume hydrogen at lower pressures.
Purpose of the Study:
- To investigate the minimum hydrogen partial pressure required for methanogenic consumption.
- To evaluate the validity of the threshold model for methane production from hydrogen in sediments.
- To reconcile conflicting findings regarding hydrogen utilization by methanogens.
Main Methods:
- Utilized pure cultures of methanogenic microorganisms.
- Controlled and measured hydrogen partial pressures during incubation.
- Monitored hydrogen consumption rates.
Main Results:
- Methanogenic isolates demonstrated no hydrogen consumption below 6.5 Pa.
- This indicates a clear minimum threshold for hydrogen utilization.
- Results contrast with a previous report suggesting lower consumption thresholds.
Conclusions:
- Pure-culture data support the existence of a threshold model for methane production.
- The threshold model remains a valid explanation for methane formation from hydrogen in sediments.
- Previous conflicting reports may be due to experimental differences or interpretations.