Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surface-active agents from two bacillus species.

D G Cooper1, B G Goldenberg

  • 1Department of Chemical Engineering, McGill University, Montreal, Quebec, H3A 2A7 Canada.

Applied and Environmental Microbiology
|February 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leucocytic Changes after the Injection of Peptone, with Particular Reference to the Arneth Count.

Edinburgh medical journal·2018
Same author

A Case in Which Von Pirquet's Reaction Was Followed by Ascites.

The Indian medical gazette·2017
Same author

Outcome in Men with a Screen-detected Abdominal Aortic Aneurysm Who are not Fit for Intervention.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2015
Same author

Thermal lensing in silver gallium selenide parametric oscillator crystals.

Applied optics·2010
Same author

Mid-IR absorption in AgGaSe(2) optical parametric oscillator crystals.

Applied optics·2010
Same author

The effect of structure and a secondary carbon source on the microbial degradation of chlorophenoxy acids.

Chemosphere·2010
Same journal

A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

Applied and environmental microbiology·2026
Same journal

Sources and traits of bacteria and fungi found in the near-surface atmosphere.

Applied and environmental microbiology·2026
Same journal

Advancing One Health in Africa through continental early warning environmental surveillance.

Applied and environmental microbiology·2026
Same journal

Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

Applied and environmental microbiology·2026
Same journal

Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

Applied and environmental microbiology·2026
Same journal

Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

Applied and environmental microbiology·2026
See all related articles

Two Bacillus species produce distinct bioemulsifiers. One yields high amounts of a neutral lipid biosurfactant, while the other creates a polysaccharide-monoglyceride complex with unique properties.

Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Microbial bioemulsifiers are crucial for various industrial applications.
  • Understanding the diversity of bioemulsifier structures and functions is essential for optimizing their use.
  • Bacillus species are known producers of various bioactive compounds, including biosurfactants.

Purpose of the Study:

  • To characterize and compare the bioemulsifiers produced by two distinct Bacillus species.
  • To investigate the chemical nature and properties of the identified bioemulsifiers.
  • To explore the relationship between different bioemulsifier components.

Main Methods:

  • Cultivation of Bacillus sp. strain IAF 343 and Bacillus cereus IAF 346 on water-soluble substrates.
  • Extraction and purification of extracellular biosurfactants.

Related Experiment Videos

  • Characterization of biosurfactant properties, including emulsifying activity and surface tension reduction.
  • Chemical analysis to determine the composition of bioemulsifiers (e.g., lipid, polysaccharide, glucosamine).
  • Ultrafiltration techniques for separating associated bioemulsifier components.

    • Main Results:

    • Bacillus sp. strain IAF 343 produced high yields (1 g/liter) of an extracellular neutral lipid biosurfactant with significant emulsifying properties but minimal surface tension reduction.
    • Bacillus cereus IAF 346 produced a polysaccharide bioemulsifier containing glucosamine, associated with a monoglyceride biosurfactant that lowered surface tension to 28 mN/m.
    • The monoglyceride and polysaccharide components from B. cereus IAF 346 formed a stable complex, requiring ultrafiltration for separation, and the polysaccharide precipitated upon monoglyceride removal.
    • The findings suggest that previously reported biopolymers with dual emulsifying and surface tension-lowering capabilities might be similar lipid-bioemulsifier aggregates.

    Conclusions:

    • Different Bacillus species can produce structurally and functionally distinct bioemulsifiers.
    • The neutral lipid biosurfactant from Bacillus sp. strain IAF 343 offers a high-yield option for emulsification.
    • The complex formed between polysaccharide and monoglyceride in Bacillus cereus IAF 346 highlights synergistic interactions and challenges in component separation.
    • This study provides insights into the diversity of microbial biosurfactants and the potential for specific applications based on their unique properties.