Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression Vector for Zymomonas mobilis.

T Conway1, M O Byun, L O Ingram

  • 1Department of Microbiology and Cell Science University of Florida, Gainesville, Florida 32611.

Applied and Environmental Microbiology
|February 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Late diagnosis, delayed presentation and late presentation among persons enrolled in a clinical HIV cohort in Ontario, Canada (1999-2013).

HIV medicine·2018
Same author

Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50°C.

Bioresource technology·2015
Same author

Removing chiral contamination of lactate solutions by selective metabolism of the D-enantiomer.

Biotechnology letters·2015
Same author

Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process.

Bioresource technology·2013
Same author

Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate.

Applied and environmental microbiology·2011
Same author

Injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by Escherichia coli MM170.

Bioresource technology·2011
Same journal

High-throughput viral enumeration of aquatic ecosystems via flow cytometry.

Applied and environmental microbiology·2026
Same journal

Gut microbiota as key mediators of animal acclimation to temperature changes: mechanisms and interventions.

Applied and environmental microbiology·2026
Same journal

Assessing the effects of ocean alkalinity enhancement on marine protozoa: physiological dynamics and transcriptomic responses.

Applied and environmental microbiology·2026
Same journal

The <i>Streptococcus mutans</i> collagen-binding protein Cnm enhances early biofilm formation with <i>Candida albicans</i>.

Applied and environmental microbiology·2026
Same journal

Response of <i>Zostera japonica</i> rhizosphere bacteria to ocean acidification.

Applied and environmental microbiology·2026
Same journal

Dynamics of clinical <i>Klebsiella pneumoniae</i> strains over the COVID-19 pandemic in Qingdao, China.

Applied and environmental microbiology·2026
See all related articles

Researchers developed new cloning vectors for efficient gene transfer into Zymomonas mobilis. These vectors enhance conjugation frequency and stability, aiding genetic engineering in bacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Efficient genetic manipulation of Zymomonas mobilis is crucial for its application in biotechnology.
  • Existing conjugation methods often suffer from low efficiency, hindering genetic studies and engineering.

Purpose of the Study:

  • To construct and characterize novel cloning vectors for high-frequency conjugation into Zymomonas mobilis.
  • To improve gene transfer efficiency in gram-negative bacteria with low conjugation rates.

Main Methods:

  • Construction of cloning vectors incorporating broad-host-range replicons (RSF1010) and multiple mob sites (RSF1010, RP4).
  • Introduction of selectable markers (chloramphenicol acetyltransferase, tetracycline resistance) and unique cloning sites (BamHI).
  • Assessment of conjugation efficiency from Escherichia coli to Zymomonas mobilis and plasmid stability in Z. mobilis.

Related Experiment Videos

Main Results:

  • Developed vectors achieving high conjugation frequencies (approaching 10 per donor/recipient) into Z. mobilis.
  • Demonstrated stable maintenance of plasmids in Z. mobilis for over 50 generations without selection.
  • Identified a Z. mobilis promoter that drives efficient beta-galactosidase expression in both Z. mobilis and E. coli.

Conclusions:

  • The engineered vectors significantly enhance conjugation efficiency into Z. mobilis.
  • These vectors offer a stable and efficient tool for genetic engineering of Z. mobilis and potentially other gram-negative bacteria.
  • The characterized Z. mobilis promoter provides a valuable tool for gene expression studies in diverse bacterial hosts.