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A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation

Ian B Powell1, Marc G Achen, Alan J Hillier

  • 1Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, and Dairy Research Laboratory, Division of Food Research, Commonwealth Scientific and Industrial Research Organization, Highett, Victoria 3190, Australia.

Applied and Environmental Microbiology
|March 1, 1988
PubMed

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
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