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Related Experiment Videos

Trichloroethylene biodegradation by a methane-oxidizing bacterium.

C D Little1, A V Palumbo, S E Herbes

  • 1Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831; Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306 ; and Environmental Engineering Science, California Institute of Technology, Pasadena, California 91125.

Applied and Environmental Microbiology
|April 1, 1988
PubMed
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This summary is machine-generated.

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A novel bacterium degrades trichloroethylene (TCE), a persistent groundwater pollutant. This methane-oxidizing microbe offers a promising biological solution for TCE remediation in contaminated water sources.

Area of Science:

  • Environmental Microbiology
  • Bioremediation
  • Environmental Chemistry

Background:

  • Trichloroethylene (TCE) is a widespread groundwater contaminant and suspected carcinogen.
  • TCE is recalcitrant to conventional aerobic biodegradation methods.
  • Effective bioremediation strategies for TCE are urgently needed.

Purpose of the Study:

  • To isolate and characterize a microbial agent capable of degrading TCE.
  • To investigate the mechanism and efficiency of TCE biodegradation by methanotrophic bacteria.
  • To assess the potential of methanotrophs for in-situ groundwater remediation.

Main Methods:

  • Isolation of an aerobic, methane-oxidizing bacterium (Strain 46-1).
  • Cultivation of Strain 46-1 on methane or methanol as growth substrates.

Related Experiment Videos

  • Analysis of TCE degradation products using gas chromatography and 14C radiotracer techniques.

    • Main Results:

    • Strain 46-1 effectively degraded TCE in pure culture at environmentally relevant concentrations.
    • TCE biodegradation was dependent on the active metabolism of methane or methanol (cometabolism).
    • Degradation yielded CO2 and water-soluble products, including dichloroacetic and glyoxylic acids.

    Conclusions:

    • Methanotrophic bacteria, specifically Strain 46-1, demonstrate significant potential for TCE bioremediation.
    • Cometabolic degradation via TCE epoxide formation is the proposed mechanism.
    • This finding supports the development of biological treatment strategies for TCE-contaminated groundwater.