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Medium for Screening Leuconostoc oenos Strains Defective in Malolactic Fermentation
1ENS.BANA, Laboratoire de Microbiologie, Université de Bourgogne, Campus Universitaire Montmuzard, 21000 Dijon, France.
Applied and Environmental Microbiology
|March 1, 1989
Summary
Researchers developed a new medium to identify Leuconostoc oenos strains lacking malolactic fermentation. This method aids in isolating mutagenic strains by differentiating colony colors based on acid production.
Area of Science:
- Microbiology
- Food Science
- Fermentation Technology
Background:
- Leuconostoc oenos is crucial for malolactic fermentation in winemaking.
- Identifying strains with defective malolactic fermentation is essential for quality control and strain improvement.
- Mutagenic strains can impact fermentation efficiency and wine characteristics.
Purpose of the Study:
- To develop a sensitive and selective medium for screening and isolating mutagenic Leuconostoc oenos strains.
- To differentiate between wild-type and defective malolactic fermentation strains.
Main Methods:
- A novel screening medium was formulated with fructose, l-malic acid, bromocresol green (pH indicator), and cellulose powder.
- The medium's efficacy was tested by observing colony color changes in response to malolactic activity.
Main Results:
- Wild-type Leuconostoc oenos colonies exhibited a blue color, indicating successful malolactic fermentation.
- Mutagenic strains with defective malolactic fermentation produced an acid reaction, resulting in yellow-green colonies.
Conclusions:
- The developed medium effectively screens and isolates Leuconostoc oenos strains defective in malolactic fermentation.
- This tool aids in the identification of specific microbial strains for research and industrial applications in winemaking.