Related Experiment Video
Updated: Aug 14, 2026
Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
Published on: December 9, 2022
Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and
J Siebert1, R J Palmer, P Hirsch
1Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, D-2300 Kiel, Federal Republic of Germany.
Abstract:
A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.
