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Related Experiment Videos

Assessment of Pediococcus acidilactici as a Potential Silage Inoculant.

A Fitzsimons1, F Duffner, D Curtin

  • 1School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, and Teagasc, Grange Research Centre, Dunsany, County Meath, Ireland.

Applied and Environmental Microbiology
|September 1, 1992
PubMed
Summary

Pediococcus acidilactici G24 is a promising silage inoculant, enhancing fermentation and improving protein content in high-carbohydrate forage. It effectively boosts lactic acid production and reduces ammonia nitrogen.

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Area of Science:

  • Agricultural Microbiology
  • Animal Science
  • Forage Preservation

Background:

  • Silage production relies on lactic acid fermentation for preservation.
  • Effective silage inoculants improve fermentation efficiency and nutritional quality.
  • Pediococcus strains are potential candidates for silage inoculation.

Purpose of the Study:

  • To screen Pediococcus strains for silage inoculant potential.
  • To evaluate the efficacy of Pediococcus acidilactici G24 as a silage inoculant.
  • To determine the influence of water-soluble carbohydrate levels on inoculant performance.

Main Methods:

  • Screening of 18 Pediococcus strains.
  • Laboratory silo experiments with grass forage.
  • Analysis of fermentation parameters (pH, lactic acid, ammonia nitrogen).

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  • Microbial population assessment (Lactobacillus plantarum).
  • Main Results:

    • Pediococcus acidilactici G24 demonstrated optimal fermentation characteristics.
    • Inoculation with P. acidilactici G24 accelerated fermentation and improved silage quality in high-WSC grass.
    • Silage inoculated with P. acidilactici G24 showed reduced ammonia nitrogen and increased crude protein.
    • Effectiveness was dependent on forage water-soluble carbohydrate (WSC) content.

    Conclusions:

    • Pediococcus acidilactici G24 is a suitable silage inoculant for forages with adequate WSC levels.
    • The inoculant enhances fermentation and nutritional value of silage.
    • Further research may optimize its application based on forage characteristics.