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Characterization of the Cricket Hindgut Microbiota with Fluorescently Labeled rRNA-Targeted Oligonucleotide Probes
J W Santo Domingo1, M G Kaufman, M J Klug
1Department of Microbiology and Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824, and Kellogg Biological Station, Hickory Corners, Michigan 49060.
Abstract:
Most cricket hindgut microorganisms (60 to 80%) were detected with a universal fluorescent rRNA-targeted probe and found to be eubacteria. Group-specific probes showed that the hindguts of five different cricket species harbor similar bacterial groups, although in different proportions, and that different diets shifted the structure of the hindgut microbial community. The Bacteroides-Prevotella probe, of the eight eubacterial probes tested, stained the largest percentage of cells in all crickets.
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