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Related Experiment Videos

Improved medium for lactic streptococci and their bacteriophages.

B E Terzaghi1, W E Sandine

  • 1New Zealand Dairy Research Institute, Palmerston North, New Zealand.

Applied Microbiology
|June 1, 1975
PubMed
Summary

Beta-disodium glycerophosphate (GP) supplementation in M17 medium enhances lactic streptococci growth and acid production. This improved medium aids in isolating lactose-negative mutants and distinguishing bacteriophages in lactic streptococci research.

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Area of Science:

  • Microbiology
  • Food Science
  • Bacteriology

Background:

  • Lactic streptococci are crucial in dairy fermentation.
  • Optimizing growth media is essential for studying these bacteria and their associated phages.
  • Beta-disodium glycerophosphate (GP) is a potential growth-enhancing supplement.

Purpose of the Study:

  • To evaluate the efficacy of beta-disodium glycerophosphate (GP) in a complex medium (M17) for lactic streptococci.
  • To assess the utility of M17 medium for isolating bacterial mutants and studying bacteriophages.
  • To investigate the impact of GP on acid production in lactic streptococci.

Main Methods:

  • Formulation of M17 medium incorporating 1.9% beta-disodium glycerophosphate (GP).
  • Comparative growth studies of Streptococcus cremoris, S. diacetilactis, and S. lactis in M17 versus control media.

Related Experiment Videos

  • Assessment of M17 medium for isolating lactose-fermenting mutants and demonstrating bacteriophage plaques.
  • Evaluation of GP-supplemented skim milk for enhanced acid production.

    • Main Results:

    • M17 medium with GP significantly improved the growth of all tested lactic streptococci strains compared to media without GP.
    • Enhanced buffering capacity of M17 medium prevented pH drops below 5.70, promoting sustained growth.
    • M17 medium facilitated the isolation of lactose-negative mutants and improved visualization of bacteriophage plaques.
    • GP incorporation into skim milk enhanced acid-producing activity of lactic streptococci.

    Conclusions:

    • Beta-disodium glycerophosphate (GP) supplementation in M17 medium is highly effective for lactic streptococci cultivation.
    • M17 medium offers advantages for bacterial mutant isolation and bacteriophage characterization.
    • GP enhances acid production in lactic streptococci, with implications for dairy fermentation processes.