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Updated: Aug 11, 2026
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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
Published on: January 17, 2012
Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors
Vladimir Khazak1, Erica A Golemis, Lutz Weber
1NexusPharma, Langhorne, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2005
Abstract:
A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
