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Updated: Aug 14, 2026
Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
Published on: January 25, 2017
Helium-neon and nitrogen laser irradiation accelerates the phagocytic activity of human monocytes
Nanda Hemvani1, Dhananjay Sadashiv Chitnis, Nijram Satramdas Bhagwanani
1Department of Microbiology and Immunology, Choithram Hospital & Research Centre, Indore, India.
Objective:
Intracellular survival of mycobacteria within monocytes is a crucial stage in the pathogenesis of tuberculosis. The aim was to check intracellular survival of Mycobacterium fortuitum within the human monocytes exposed to He-Ne and nitrogen laser irradiation.
Background Data:
Tuberculosis remains one of the most important infectious diseases for developing countries. Low-level laser therapy (LLLT) has been tried to treat tubercular cavitory lung disease with encouraging results. The in vitro photobiological effect of low level laser radiation on the intracellular mycobacteria needs to be evaluated before we could go for large clinical trials.
Methods:
The aliquots of human monocytes from peripheral blood of healthy volunteers and tuberculosis cases were exposed to He-Ne or nitrogen laser beam. The non-irradiated monocytes from the same source served as controls. The monocytes were then challenged with M. fortuitum, and surviving mycobacteria within monocytes were subjected to viable counts.
Results:
Enhanced killing of mycobacterial cells was seen among monocytes exposed to He-Ne and nitrogen laser irradiation.
Conclusions:
He-Ne and nitrogen laser irradiation activates the monocytes to increase intracellular killing of mycobacteria.
