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Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

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Differential beta-arrestin binding of AT1 and AT2 angiotensin receptors

Gábor Turu1, László Szidonya, Zsuzsanna Gáborik

  • 1Department of Physiology, Semmelweis University, Faculty of Medicine, H-1444 Budapest, P.O. Box 259, Budapest, Hungary.

FEBS Letters
|December 20, 2005
PubMed

Agonist stimulation of G protein-coupled receptors causes receptor activation, phosphorylation, beta-arrestin binding and receptor internalization. Angiotensin II (AngII) causes rapid internalization of the AT1 receptors, whereas AngII-bound AT2 receptors do not internalize. Although the activation of the rat AT1A receptor with AngII causes translocation of beta-arrestin2 to the receptor, no association of this molecule with the AT2 receptor can be detected after AngII treatment with confocal microscopy or bioluminescence resonance energy transfer. These data demonstrate that the two subtypes of angiotensin receptors have different mechanisms of regulation.

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