Related Experiment Video
Updated: Aug 14, 2026
Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
Published on: July 12, 2022
Quantification of mitochondrial DNA using real-time polymerase chain reaction in patients with premature ovarian
Kwang-Yul Cha1, Sook-Hwan Lee, Hyung-Min Chung
1Department of Obstetrics and Gynecology, CHA General Hospital, Pochon CHA University, Seoul, Korea.
Objective:
To quantify mitochondrial DNA using real-time PCR in women with premature ovarian failure (POF) and a control group.
Design:
Prospective study.
Setting:
Genome Research Center for Reproductive Medicine and Infertility, Korea Ministry of Health & Welfare.
Patient(S):
Thrity patients with POF and 30 control individuals.
Intervention(S):
The mitochondrial DNA content was quantified using real-time PCR. The effectiveness of the assay was determined by relative quantification using the comparative threshold cycle (CT) method.
Main Outcome Measure(S):
Relative quantification of mitochondrial DNA content.
Result(S):
The mitochondrial DNA content was significantly lower in the POF group than in the control group (0.58 +/- 0.38 vs. 1.15 +/- 0.67; P < .01). In both groups, there was a significant positive correlation between the mitochondrial DNA/28S rRNA ratio and mitochondrial DNA CT (control group: r = 0.774; P < .001; POF group: r = 0.556; P = .001) and a significant negative correlation between the mitochondrial DNA/28S rRNA ratio and 28S rRNA CT (control group: r = -0.677; P < .001; POF group: r = -0.627; P = .001).
Conclusion(S):
This study has established the clinical feasibility of quantifying amounts of mitochondrial DNA, relative to an internal standard, using real-time PCR. Further studies are warranted to elucidate the roles of apoptosis and mitochondrial function in the pathogenesis of POF.
Related Concept Videos
02:57Real Time RT-PCR
The real-time quantification of the number of amplified products is...
02:59Animal Mitochondrial Genetics