Related Experiment Video

Updated: Aug 14, 2026

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

Development of an internally quenched fluorogenic substrate for kinetic analysis of thioesterases

Zachary Q Beck1, David H Sherman

  • 1Life Sciences Institute, Departments of Medicinal Chemistry, Chemistry, Microbiology, and Immunology, University of Michigan, Ann Arbor, MI 48103, USA.

Analytical Biochemistry
|December 20, 2005
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination
16:02

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination

Published on: February 21, 2013

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

Related Experiment Videos

Last Updated: Aug 14, 2026

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination
16:02

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination

Published on: February 21, 2013

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

Related Concept Videos

Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

Articles linked to this work by shared authors, journal, and citation graph.

Regulator-Guided Strain Prioritization and Genome Engineering Uncovers Concanamycin Analogs.

Journal of natural products·2026

Large-scale discovery platform enables identification of peptides targeting drug-resistant candidiasis.

Cell reports methods·2026

Chemoselective Halogenation of Premarineosin A for Next-Generation Antimalarial Development.

bioRxiv : the preprint server for biology·2026

Investigating Opioid Receptor Activity through Biocatalytic Halogenation and Oxidation of Mitragynine.

ACS chemical biology·2026

Structural Diversification of 14-Membered Macrolides by Chemoenzymatic Synthesis.

JACS Au·2026

Diverse Cyanopeptides follow distinct temporal succession patterns in freshwater harmful algal blooms.

The ISME journal·2026

Analytical performance comparison of Siemens Atellica CI1900 and Roche Cobas Pure integrated analyzers across spectrophotometric, ion-selective electrode and immunoassay parameters.

Analytical biochemistry·2026

Development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify protoporphyrin IX (PPIX) and zinc (II) protoporphyrin IX (Zn-PPIX), in human plasma.

Analytical biochemistry·2026

Voltammetric study of doxorubicin interaction with single-stranded and double-stranded DNA.

Analytical biochemistry·2026

A dual signal ratio electrochemical sensor based on DNA tetrahedrons and bimetallic nanocomposites for AFB1 analysis.

Analytical biochemistry·2026

An enzyme-free HCR-amplified colorimetric biosensor based on a Cu2+/rGO nanozyme for detection of dengue virus.

Analytical biochemistry·2026

Rapid identification of dried suckling deer using molecular authentication-based PCR-GICA and PCR-FQICA.

Analytical biochemistry·2026

Sustainable stability-indicating spectra manipulations for the concurrent quantification of gliclazide and its impurity A as the possible alkaline degradation product: Multi-Color Analytical Profile Assessment.

Scientific reports·2026

Response to volatiles from decaying organic matters and behavioural plasticity in male and female Hermetia illucens.

Journal of insect physiology·2026

Extended blood ethyl glucuronide persistence after controlled ethanol administration: Implications for ethanol-negative delayed DUI cases.

Forensic science international·2026

Ion mobility mass spectrometry: A unique platform for glycan isomer characterization.

Journal of chromatography. A·2026

Chimeric ascorbic acid phosphate-releasing nucleotide probes for isothermal signal-amplified protein detection.

Talanta·2026

Carambola-like metal-AIEgen framework-based fluorescent lateral flow assay for the sensitive detection of zearalenone.

Mikrochimica acta·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us