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Updated: Aug 14, 2026

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

[Expression and function of receptors for advanced glycation end products in bovine corneal endothelial cells]

Yuichi Kaji1

  • 1University of Tsukuba, Institute of Clinical Medicine, Department of Ophthalmology, 1-1-1 Tennoudai, Tsukuba 305-8575, Japan. kajiyuichi@hotmail.com

Nippon Ganka Gakkai Zasshi
|December 21, 2005
PubMed

Insights

Advanced glycation end product (AGE) interactions with corneal receptors contribute to age-related corneal endothelial cell loss. AGE receptor (RAGE) and galectin-3 play distinct roles in this process.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Aging Research

Background:

  • Corneal endothelial cell loss is a common age-related change with an unclear mechanism.
  • Advanced glycation end products (AGEs) are implicated in cellular aging and damage.

Purpose of the Study:

  • To investigate the role of AGE-receptor interactions in age-related corneal endothelial cell loss.
  • To examine the expression and function of AGE receptors (RAGE and galectin-3) in bovine corneal endothelial cells.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) to detect gene expression.
  • Immunohistochemistry to visualize protein localization.
  • In vitro studies on cultured corneal endothelial cells exposed to AGEs.

Main Results:

  • RAGE and galectin-3 were expressed in bovine corneal endothelial cells.
  • Galectin-3 mediated AGE internalization.
  • RAGE was involved in reactive oxygen species generation and apoptosis induction.

Conclusions:

  • AGE-receptor interactions are a potential mechanism for age-related corneal endothelial cell loss.
  • RAGE and galectin-3 play differential roles in AGE-induced cellular responses.
  • Targeting these pathways may offer therapeutic strategies for age-related corneal changes.

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