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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
Published on: August 30, 2017
A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement
Yi Xiao1, Brian D Piorek, Kevin W Plaxco
1Department of Physics, Institute for Polymers and Organic Solids, Program in BioMolecular Science and Engineering, University of California, Santa Barbara, California 93106, USA.
Abstract:
Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as approximately 3 nM.
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