Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultrahigh pressure liquid chromatography using elevated temperature.

Yanqiao Xiang1, Yansheng Liu, Milton L Lee

  • 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602-5700, USA.

Journal of Chromatography. A
|December 27, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting NEK9 synergises with immunotherapy in hepatocellular carcinoma by remodelling the immunosuppressive microenvironment.

Gut·2026
Same author

A causal link between autoantibodies and neurological symptoms in long COVID.

Cell·2026
Same author

PI3K Regulates Wild-type RAS Signaling to Confer Resistance to KRAS Inhibition.

Cancer research·2026
Same author

A Potassium Phosphoramidate-Based Strategy for Compiling and Profiling the Noncanonical <i>N</i>-Phosphoproteome of Nasopharyngeal Carcinoma.

Analytical chemistry·2026
Same author

Baseline cellular state dictates the molecular impact of <i>KRAS</i> mutant variants in pancreatic cancer cells.

bioRxiv : the preprint server for biology·2026
Same author

A Deep Quantitative Proteome Turnover Platform for Human iPSC-derived Neurons.

bioRxiv : the preprint server for biology·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
Same journal

Stereo-sensitive modelling of gas chromatographic retention indices of mono-cycloalkanes in jet fuel range.

Journal of chromatography. A·2026
Same journal

Approaches to using retention indices with coupled column pressure tuning in gas chromatography.

Journal of chromatography. A·2026
Same journal

MOF-supported surface-imprinted polymer for hazard governance of aristolochic acids in herbal matrices: A safety-control strategy supported by multiscale simulations.

Journal of chromatography. A·2026
See all related articles

Achieve fast liquid chromatography (LC) separations by combining ultrahigh pressures with elevated temperatures. This method uses small particles for high efficiency, completing separations in seconds with minimal loss of column performance.

Area of Science:

  • Analytical Chemistry
  • Chromatography Science

Background:

  • Fast liquid chromatography (LC) is crucial for various applications.
  • Reducing particle diameter (d(p)) enhances separation speed and efficiency.
  • Small particles (<2 µm) necessitate ultrahigh pressures (>689 bar) due to flow resistance.

Purpose of the Study:

  • To demonstrate fast and efficient LC separations using elevated temperatures and ultrahigh pressures.
  • To investigate the impact of combining high temperature and pressure on separation performance.

Main Methods:

  • Utilized C6-modified 1.0 µm nonporous silica particles.
  • Operated at an elevated column temperature of 80°C.
  • Employed ultrahigh pressures up to 2413 bar.

Related Experiment Videos

Main Results:

  • Achieved rapid separations completed in as little as 30 seconds.
  • Obtained high column efficiencies reaching 220,000 plates/m.
  • Demonstrated that elevated temperature reduces mobile phase viscosity, enabling higher flow rates.

Conclusions:

  • Combining elevated temperature (80°C) with ultrahigh pressure (2413 bar) facilitates rapid and efficient LC separations.
  • This approach allows for significantly shortened separation times with minimal loss in column efficiency.
  • The use of small particle sizes (1.0 µm) combined with optimized conditions yields exceptional chromatographic performance.