Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Actions of ultraviolet light on cellular structures.

David I Pattison1, Michael J Davies

  • 1The Heart Research Institute, 145 Missenden Rd, Camperdown, NSW 2050, Australia. d.pattison@hri.org.au

EXS
|December 31, 2005
PubMed
Summary

Ultraviolet (UV) radiation causes cellular damage to DNA and proteins through direct absorption or photosensitization, leading to mutations and skin cancer. Understanding these mechanisms is crucial for developing effective sun protection strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing a cost and time efficient strategy for assessing novel materials for wastewater treatment with non-target screening analysis.

Journal of hazardous materials·2026
Same author

Fermenting shea nuts using the traditional pit method yields better physicochemical properties with potential environmental benefits.

Scientific reports·2025
Same author

New strategies for non-targeted quantification in comprehensive two-dimensional gas chromatography: The potential of reconstructed TIC response factor surfaces.

Journal of chromatography. A·2025
Same author

Redox factors in the antioxidant activity of nitroxides toward DNA guanyl and 2-deoxyribose-peroxyl radicals.

Free radical research·2024
Same author

Comprehensive two-dimensional gas chromatography as a tool for targeted and non-targeted analysis of contaminants of emerging concern in wastewater.

Talanta·2024
Same author

System stability and signal enhancement with analyte protectants: Gas chromatography analysis of oxygenated-polycyclic aromatic hydrocarbons.

Talanta·2024

Area of Science:

  • Biochemistry
  • Photobiology
  • Molecular Biology

Background:

  • Solar radiation is the main source of human exposure to ultraviolet (UV) radiation.
  • Overexposure to UV radiation without protection can lead to mutagenesis and skin cancer.
  • Cellular damage, primarily to DNA and proteins, is initiated by UV radiation.

Purpose of the Study:

  • To review the mechanisms of UV-mediated cellular damage.
  • To discuss the formation of UV-induced DNA and protein modifications.
  • To highlight the downstream consequences of these modifications, including mutagenesis and altered protein structure/function.

Main Methods:

  • Review of existing literature on UV radiation effects.
  • Discussion of direct UV absorption and photosensitization pathways.

Related Experiment Videos

  • Analysis of DNA and protein damage products and their formation.

    • Main Results:

    • UV radiation induces DNA damage via direct absorption, forming dimers (cyclobutane pyrimidine, pyrimidine (6-4) pyrimidone, Dewar isomers), and via photosensitization, producing 8-oxo-Gua.
    • Protein damage is predominantly mediated by singlet oxygen ((1)O2) via photosensitization, affecting amino acid residues like Trp, His, Tyr, Met, Cys.
    • UV-induced damage results in DNA mutations (e.g., CC-->TT transversions) and protein alterations (cross-links, aggregates) impacting cellular function.

    Conclusions:

    • UV-induced DNA and protein damage are significant contributors to mutagenesis and skin carcinogenesis.
    • Understanding the distinct mechanisms of direct absorption and photosensitization is key to comprehending UV damage.
    • Further research into these modifications and their consequences is vital for developing effective protective measures and therapeutic strategies.