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Related Experiment Videos

Cell surface changes and Rous sarcoma virus gene expression in synchronized cells.

A H Hale, J L Winkelhake, M J Weber

    The Journal of Cell Biology
    |February 1, 1975
    PubMed
    Summary
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    Cell surface changes linked to cancer growth are not tied to the cell cycle. Tumor virus gene expression can alter cell membrane function at any cell cycle stage.

    Area of Science:

    • Cell Biology
    • Cancer Research
    • Virology

    Background:

    • Cell surface alterations are associated with growth control and malignant transformation.
    • Understanding the relationship between these changes and the cell cycle is crucial for cancer research.

    Purpose of the Study:

    • To investigate the link between cell cycle progression and cell surface changes in normal and transformed cells.
    • To examine how tumor virus gene expression affects cell membrane function throughout the cell cycle.

    Main Methods:

    • Synchronization of chicken embryo cells using a double thymidine block.
    • Assessment of cell-cycle-dependent membrane function via nutrient transport assays (2-deoxyglucose, uridine, thymidine, mannitol).
    • Evaluation of cell surface morphology using scanning electron microscopy.

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    Main Results:

    • Normal growing cells do not exhibit the high 2-deoxyglucose transport rates seen in transformed cells or the low rates characteristic of density-inhibited cells.
    • Cell cycle-dependent changes in cell surface morphology were observed but did not correlate with membrane function alterations.
    • Tumor virus gene expression altered cell membrane function irrespective of the cell cycle stage or progression.

    Conclusions:

    • Cell cycle progression does not dictate the cell surface changes associated with malignant transformation.
    • Membrane function alterations induced by tumor viruses are independent of the cell cycle stage.
    • Cell cycle-dependent morphological changes do not directly translate to functional membrane changes.