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Updated: Aug 13, 2026
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
Published on: August 21, 2011
Neurite outgrowth promoting effects of enriched and mixed OEC/ONF cultures
Ronald Deumens1, Guido C Koopmans, Marijke Lemmens
1Department of Psychiatry and Neuropsychology, Division Neuroscience, European Graduate School of Neuroscience (EURON), Maastricht University, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. r.deumens@np.unimaas.nl
Abstract:
Olfactory ensheathing cell (OEC) transplants stimulate axon regeneration and partial functional recovery after spinal cord injury. However, it remains unclear whether enriched OEC or mixed transplants of OEC and olfactory nerve fibroblasts (ONF) are optimal for stimulating axon regrowth. The neurite outgrowth stimulating effects of enriched OEC, ONF, and mixed OEC/ONF cultures on neonatal cerebral cortical neurons were compared using co-cultures. We show that (1) OEC are more neurite outgrowth promoting than ONF, and (2) ONF do not enhance the neurite outgrowth stimulating effects of OEC in mixed OEC/ONF cultures. Hence, our data indicate that there is no preference for the use of enriched OEC or mixed OEC/ONF cultures with respect to stimulation of neurite growth in vitro.
